****
Just got screwed.. Damn shit.. Hai.. Ultra sad... Used the wrong cells so the protein of interest cant be expressed.. Hai.. But I didnt know.. Blah Blah told me to use DH5alpha and so I did.. Apparently he didnt tell me that DH5alpha is for plasmid amplification and not protein expression.. And here I am thinking that DH5alpha is the cheaper version of BL23 CodonPlus.. Hai...
Got questioned by Jimmy.. Sian shit..
But well at least Dr Cheong an wei me..
And Grant appologise for not telling me.. Hai..
But still sian shit.. Being said by your supervisor..
And now I have to repeat my expt..
Its going to cost me one week.. (5days)
Thats a lot of time..
Damn....
Damn....
Damn....
